If you have a NetAPP filer you can use their tool: mbralign.
Check if your storage vendor offers a best practice document regarding alignment (at least EMC and NetAPP does).
Read this document, it contains some instructions on how to achieve alignment for Windows and Linux guests: http://www.vmware.com/pdf/esx3_partition_align.pdf
Microsoft offers several document and some tools. Go to their site and search for "align partition".
AWo
VCP 3 & 4
Author @ vmwire.net
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