Hello irfan.mugale ,
If you change the private key of gateway and portal, yes, you need to redo the integration between portal and gateway.
for gateway,
you need to import the new private key, and mark it as default ssl key (Set a Default SSL or CA Private Key - CA API Gateway - 9.2 - CA Technologies Documentation )
for portal,
if you want to enable SSL with the new private key, Configure SSL for the API Portal - CA API Developer Portal - 3.5 - CA Technologies Documentation
for redo integration,
on portal, you install the new portal private key and new gateway public key, on gateway, you need to import new portal public key, and create new user associated to the portal public key (user name = portal public key CN)
If you have any problem, you're welcome to open a support ticket.
Regards,
Mark