Hello,
The Symantec Endpoint Recovery Tool (SERT) is a bootable CD that can scan and remove malware from an infected computer.
SERT is useful in situations where computers are too heavily infected for the Symantec Endpoint Protection client installed upon them to clean effectively.
It is also necessary against specific threats which have the ability to completely hide from Windows, or that have techniques that manipulate Windows into protecting the malicious process against Symantec Endpoint Protection's scanning and remediation components.
Yes, it is possible to use it by a pen drive without CD or DVD and
Yes, it supports Windows XP Service Pack 2 or later, Windows Vista and Windows 7
Check this Articles:
How to make the Symantec Endpoint Recovery Tool boot from a USB memory stick
How To Use the Symantec Endpoint Recovery Tool with the Latest Virus Definitions
Symantec Endpoint Recovery Tool (SERT) download comes as an ISO (disk image). How do I use this?
What does the full scan from the Symantec Endpoint Recovery Tool (SERT) CD scan ?
Hope that helps!!