, two main points here:
MTU: if you change MTU to something mismatched or higher than switch/end-to-end supports, vSAN performance will degrade horribly, BUT, it won't isolate the nodes...until you reboot (or anything that causes leave+rejoin). The prudent action would have been to just set this back to 1500 on the vmk.
vSAN cluster & vsanDatastore have a 1:1 relationship: you have probably created a new vSAN cluster here and thus a new vsanDatastore - your OSFS path of all objects is probably now incorrect.
This can be fixed in 2 ways:
1. Rejoin the original cluster with the Sub-cluster UUID - if you don't have persistent logging then this can be pulled from an old log bundle or other resource. If you do have logs from before reboot then check vsansystem.log.
2. Change all Objects OSFS path to reference the new vsanDatastore ID instead of old.
While this issue was caused by human error, please don't worry about it, if you don't understand how to action the above, please open a case with vSAN GS so my colleagues can assist with 1./2. .